Contact an Account ManagerAutomated in-needle derivatization applying a user-defined program for the Thermo Scientific Dionex WPS-3000 split-loop autosampler
Summary
Type: HPLC
Application #: 126180
Detection:
Conditions: Eluent A: 10 mmol/L Na2HPO4, 10 mmol/L Na2B4O7 · 10 H2O, adjusted to pH 7.8 with HCl (fuming)
Eluent B: acetonitrile – methanol – water (45:45:10, v/v/v)
Gradient: 2-10 % B in 0.1 min, 10-20 % B in 1.4 min, 20-38 % B in 5.0 min, 38-57 % B in 1.7 min, 57-100 % B in 0.1 min, 100 % B for 1.7 min, 100-2 % B 0.5 min, 2 % B for 5.5 min
Flow rate: 0.7 mL/min
Temperature: 40 °C
Injection volume: The volume is defined by UDP settings. 10 µL is injected onto the
column, originating from a drawn sample volume of 1 µL.
Authors: Dionex
Source: Thermo Scientific Dionex, technical note 107